Abstract
Summary - Ptaquiloside has been discovered for the first time in milk samples 01 a bovine fed a dietary complement (6 kg fresh weighVday) of freshly-eut bracken fronds. This was equivalent to ca 216 mg/day pure ptaquiloside. The compound was found in a quantity of no less than 0.11 mg/I in milk. This was determined by the appearance of pterosin 6 - the decomposition product of ptaquiloside - in prepurified milk sampi es treated with milg 1I1kllii (pH 8.0 and 11.5). The lack of base treatment led to the absence of pterosin B. A mi!kj}rilpurlflcatlon protocol based on meth,anol-acetonitrile precipitations in succession, hexllne and dlchloromethane extractions, alkaline treatment, reextraction and silica gel microcolumn chromatography followed by reverse-phase HPLC was developed for the detection 01 ptaquiloside.
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