Abstract
MS2 bacteriophage has a reported nominal molecular weight of 2M daltons. It would be expected that this phage would not pass through filters of various sizes with low molecular weight cutoff (MWCO) values of less than 1M daltons. It was discovered that MS2 bacteriophage will pass through filters with 750K-, 500K-, and 300K-dalton MWCO values. MS2 was retained on the 100K-dalton filter. A cross-flow hollow fiber apparatus was used for the 750K- and 500K-dalton analysis. Centrifuge filters of 1M and 300K and 100K daltons were used. The rate of passage of MS2 through the cross-flow filters is dependent on the tangential flow rate and pressure. Passage through the centrifuge filters depended on the gravitational force applied.
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