Abstract
Abstract The partitioning behavior of menadione-induced NAD(P)H oxidase from the white rot fungus Phanerochaete chrysosporium has been studied in various poly(ethylene glycol) (PEG)-salt aqueous two-phase systems (ATPSs). The effects of the molecular weight of the PEG, system composition, and pH of the system on NAD(P)H oxidase partitioning were investigated at 25 °C. The best partition parameters were observed in the PEG 2000 18%–K 2 HPO 4 13%–NaCl 0.5% (w/w, pH 7.0) system; the partition coefficient of total NAD(P)H oxidase activity ( K e ) of the system was 0.232. While the recovery in the bottom phase ( R b ) was 87.644%, this value was 20.350% in the top phase of the optimized system. The purification fold was found as 8.331 in the bottom phase. NAD(P)H oxidase purified from P. chrysosporium by the PEG–K 2 HPO 4 two-phase system was determined to be a homodimer in its native state with a molecular weight of 116 kDa.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
