Abstract
Two models (partition and electrostatic) were utilized to explain the retention data of four polar compounds with guanidine or tertiary amine moieties (metformin, phenformin, tolylformin, and ranitidine) in ion-pairing liquid chromatography based on C18 stationary phase (Zorbax Eclipse XDB column). Mobile phase composition, concentration of the ion-pairing (alkylsulphonates with 5 – 8 carbon atoms), and pH of the aqueous component were varied to determine the corresponding dependence of the retention factors and explanation by one model. Good correlations were found for retention factor versus concentration of ion pairing alkyl sulfonates concentration with a slope similar to the predicted values by the electrostatic model and between retention factor and the content of methanol used as organic modifier in mobile phase. Some extrapolations of the linear dependences were used in estimating the hydrophobic character of the pair between analyte and alkyl sulfonate ions in accordance to the partition model.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
