Abstract
In order to obtain cDNA clones encoding 18S ribosomal RNA or an antibacterial protein, a fat body cDNA library of the coconut rhinoceros beetle, Oryctes rhinoceros, was screened using common probes which could hybridize both cDNAs. Partial nucleotide sequencing of two positive clones revealed that both clones encode 18S rRNA but not an antibacterial protein. Comparison of the nucleotide sequence of the two positive clones with those of 18S rRNA genes from two bee species, Mesopolobus sp. and Evania appendigaster indicated that the partial sequence from O. rhinoceros is identical to those of these two bee 18S rRNA genes. This cDNA will be useful as a probe for an internal marker to explore regulatory mechanisms of antibacterial protein gene expression in O. rhinoceros.
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