Abstract
Knowledge of the Michaelis–Menten parameters and their meaning in different circumstances is an essential prerequisite to understanding enzyme function and behaviour. The published literature contains an abundance of values reported for many enzymes. The problem concerns assessing the appropriateness and validity of such material for the purpose to which it is to be applied. This review considers the evaluation of such data with particular emphasis on the assessment of its fitness for purpose.
Highlights
Cooperativity and allosteric effects can be addressed by use of a reversible model of the Hill equation [6], an extended Monod, Wyman, and Changeux allosteric equation [7], or an approximate version of these [8]
The more recently developed STRENDA (STandards for Reporting ENzymology DAta) database [12] seeks to address these problems by establishing the information that should be made available when reporting such data
A more difficult problem has been that many investigators who have been interested in the kinetic behaviour of specific enzymes have used assay conditions that are favourable to their studies without regard for the physiological conditions under which the enzyme may operate in the tissues [17]
Summary
Cooperativity and allosteric effects can be addressed by use of a reversible model of the Hill equation [6], an extended Monod, Wyman, and Changeux allosteric equation [7], or an approximate version of these [8] This will result in a chain of ODEs representing each transformation in a metabolic process (see [9], for example). The only requirement is knowledge of the kinetic parameters and behaviour of each enzyme and the transport process in the system and the rates of any non-catalysed reactions that might be involved. This seems relatively straightforward, but unless the parameters used are accurate and reliable, it will otherwise be—as stated in the quite old adage—a case of “garbage-in, garbage-out”.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
