Abstract
Mutation of the p53 protein may represent the commonest genetic event in human malignancy. Abnormal p53 expression has been reported in a variety of carcinomas, sarcomas and lymphoid neoplasms; however there is little information in relation to Hodgkin's disease. The expression of the nuclear phosphoprotein was investigated in paraffin-embedded biopsies from fifty patients with Hodgkin's disease using a polyclonal antibody, CM-1 and in snap-frozen material with monoclonal antibodies, PAb 1801 and PAb 240. Specifically, immunoreactivity was localised to the Reed-Sternberg cells or mononuclear variants in both nodular sclerosing (86% cases) and mixed cellularity (57% cases) subtypes of Hodgkin's disease. However, no positive staining was found in our cases of nodular lymphocyte predominant type Hodgkin's disease. Serial biopsies following recurrence of disease demonstrated consistent results. It is suggested that overexpression of p53, probably mutant, may have a role in the tumorigenesis of Hodgkin's disease.
Highlights
Formalin-fixed paraffin-embedded blocks of 50 cases of Hodgkin's disease (HD) were retrieved from the histopathology files and snap-frozen material from 12 cases from the tissue bank
Sections from a p53 positive colonic adenocarcinoma were used as positive controls and normal tonsil or normal lymph nodes as negative controls
Accumulation of the p53 protein is a common feature in almost all human malignancies studied
Summary
Formalin-fixed paraffin-embedded blocks of 50 cases of HD were retrieved from the histopathology files and snap-frozen material from 12 cases from the tissue bank. All the patients had been treated at St Bartholomew's Hospital and selected on the basis of availability of suitable material. Sections from a p53 positive colonic adenocarcinoma were used as positive controls and normal tonsil or normal lymph nodes as negative controls. Four micron paraffin sections were processed using the avidin-biotin complex (ABC) method (Vectastatin Elite kit, Vector, Burlingame, CA.) with diaminobenzidine tetrahydrochloride as the chromogen (Guesdon et al, 1979). Sections were incubated overnight at 4°C with CM-1 diluted 1 in 2000 in PBS. Snap-frozen material was sectioned and stained with PAb 1801 or PAb 240 using the indirect immunoperoxidase method (Filipe & Lake, eds, 1990, page 479)
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