P241 A study of immunocomplex capture fluorescence analysis using lymphocyte and monocyte fraction by freezing isolation

Abstract

Aim Immunocomplex capture fluorescence analysis (ICFA) is a novel method to predict rejection/acceptance responses in organ transplantation. The method is less susceptible to the effect of drug compared with traditional methods. The method uses fluorescent beads to sensitively detect antibodies directed against HLA I/II antigens on blood cells.In this study, the changes in mean fluorescence intensity (MFI) control values of lymphocyte and monocyte fraction from donor cells before cryopreservation and donor cells after one month of cryopreservation were monitored using ICFA for a lymphocyte crossmatch. Methods The subjects were patients who were going to undergo living-donor kidney transplantation. ICFA method for a lymphocyte crossmatch was performed. The stability of MFI control values was monitored for donor cells before cryopreservation and those after one month of cryopreservation. Results The monitoring results showed that the MFI control value of lymphocyte and monocyte fraction from donor cells after one month of cryopreservation was 9.4% higher than that of lymphocyte and monocyte fraction from donor cells before cryopreservation. On the other hand, the MIF control value of leukocytes after one month of cryopreservation using the conventional method was 100%. Conclusions More reliable donor-specific HLA antibody test results can be obtained by lymphocyte crossmatch test using lymphocyte and monocyte fraction by freezing isolation from cryopreserved donor cells.