Abstract
traditional herbal recipes (THR-SK004, THR-SK010, THR-SK011) on biofilm formation and on mature biofilm of a reference strain of Pseudomonas aeruginosa . Methods: Effects of the extracts on the biofilm mass were evaluated by using crystal violet (CV) assay. The respiratory activity of preformed biofilm of Pseudomonas aeruginosa after treatment with the extract was determined by MTT reduction assay. Scanning electron microscopywas used to furnish images of biofilm reduction after the recipe treatment. Results: Tested ethanol extracts displayed anti-biofilm activity, but the water extracts exhibited low biofilm inhibition activity at the tested concentrations. Remarkable reduction in biofilm formation of P. aeruginosa was found after treatment with THR-SK010 ethanol extract (THR-SK010E). Treatments with this extract resulted in prevention of biofilm formation of P. aeruginosa on both polystyrene and glass surfaces. Almost 50% reduction in the bacterial metabolic activity in the preformed biofilm was seen after exposure to the extract-supplemented buffer for 12 h. Following a 24-h treatment with THR-SK010E at 62.5mg/mL, 97.3% of the preformed biofilms was destroyed. Conclusion: Promising anti-biofilm activity was displayed by THRSK010 ethanol extract suggesting further investigation in order to explore the possible utilization of the herbal recipe as an anti-biofilm agent, especially for wound treatment.
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