P1.19 HPV Influence on Genes Expression in Cin and Cervical Cancer

Abstract

ABSTRACT Cervical cancer is one of the most common diseases in women and a role of human papilloma virus (HPV) as the trigger of malignization is considered to be proved. Virus replication influence alterations in gene expression pattern provoking proliferative and antiapoptotic activity of infected cell - fundamental features of malignization. We analyzed KI67, Aurora A, Birc5, cyclin B1, CTSL2, MMP11, Her2/neu, GRB7, BCL2, BAG1, BAX, NDRG1, ESR, PRG, PTEN, MGB and hTERT expression by qPCR in normal HPV positive/negative cervical epithelium (CE), through cervical intraepithelial neoplasia progression (CIN I-III) and in cervical cancer (CC). Materials and methods mRNA expression level of studied genes was quantified by qPCR in 76 tissue samples taken from women with CIN I (n = 17), CIN II (n = 11), CIN III (n = 12) and CC (n = 16). Negative control group included 20 samples of morphologically normal cervical epithelium (NE). Results Expression of KI67, CCNB1 and hTERT was increased during disease progression from normal epithelium through CIN to cervical cancer, but BCL2, BAG and BAX expression was simultaneously decreased (p = 0,0120). Expression of CCNB1 (p = 0,0042), BAG1 (p = 0,012) and hTERT (p = 0,0220) is much higher in CIN III than in CIN I-II. Besides gene expression alterations allow to classify normal, CIN and cancer tissues with 75-92% specificity (p Conclusion HPV persistence changes proliferative and antiapoptotic activity of NE, CIN and CC cells in different manner. Gene expression alterations in different degree of CIN and CC may be used as a classification model for determination of high risk neoplastic lesions in clinical practice.