Abstract
Results The data show that gp120 and ICAM-1 on the bilayers can induce the formation of a synaptic structure by noninfected CD4+ T cells with some characteristics of an immunological synapse (IS). There is a transient stop signal and the interface contains a central supramolecular activation cluster (cSMAC) of gp120-CD4 interactions surrounded by a peripheral supramolecular activation cluster (pSMAC) of ICAM-1-LFA-1 interactions. As with the IS, the cSMAC is also formed from microclusters that are enriched in active Src family kinases. However, while Src kinase signaling is suppressed in the cSMAC of the IS, the Src family kinases and other TCR signaling components remain active for a prolonged period in the cSMAC of the VS. Notably, the gp120-enriched cSMAC region of the VS is depleted of f-actin.
Highlights
HIV can be transferred from cell to cell via a virological synapse (VS)
These results indicate that, in addition to its role in initiating virus infection, HIV envelope gp120 interaction with target CD4+ T cells triggers activation signals that lead to formation of an f-actin-depleted zone in the VS, which is important for viral entry
The data show that gp120 and ICAM-1 on the bilayers can induce the formation of a synaptic structure by noninfected CD4+ T cells with some characteristics of an immunological synapse (IS)
Summary
HIV gp120 interaction with CD4+ T cells induces local intracellular signaling and creates an F-actin depleted zone in the virological synapse. Address: 1Case Western Reserve University, Cleveland, OH, USA and 2Pathology, New York University School of Medicine, New York, USA * Corresponding author from AIDS Vaccine 2009 Paris, France. Published: 22 October 2009 Retrovirology 2009, 6(Suppl 3):P101 doi:10.1186/1742-4690-6-S3-P101. AIDS Vaccine 2009 Anna Laura Ross Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf
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