P046 Changes of cytokines and outcome prediction in burn patients

Abstract

Introduction Major burn injury induces an inflammatory response, which is accompanied by the release of various cytokines.This study was performed to investigate changes in levels of pro-inflammatory and anti-inflammatory cytokines following burn injury, and to investigate the relationship between levels of these cytokines and prognosis in burn patients. Methods Blood samples of 40 burn patients were collected on days 1 and 3 after burn injury, and concentrations of G-CSF, GM-CSF, INF- γ , TNF-α, IL-1β, IL-1rα, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF-BB, RANTES, VEGF, Basic FGF, and Eotaxin were measured using the Bio-Plex Suspension Array System. Blood samples of 25 healthy non-burn subjects were used as controls. Clinical data including patients’ outcome (survival or death) were collected from the patients’ medical records. Statistical differences in each cytokine concentrations between control and patient groups, between day 1 and day 3, and between survival and death groups were analyzed using the Mann-Whitney’s U -test. Results Median concentrations of G-CSF, GM-CSF, IL-1β, IL-1rα, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, MCP-1, MIP-1α, MIP-1β, and VEGF were significantly increased in burn patients compared to those in non-burn controls ( P γ , IL-1β, IL-1rα, IL-2, IL-4, IL-7, IL-9, IL-10, IL-12p70, IL-13, IL-17, MIP-1α, MIP-1β, PDGF-BB, VEGF, basic FGF, and Eotaxin were significantly higher on day 1 compared to day 3, whereas G-CSF were significantly higher on day 3 compared to day 1 ( P Conclusion These findings indicate that G-CSF, GM-CSF, IL-1β, IL-1rα, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, MCP-1, MIP-1α, MIP-1β, and VEGF play important roles in inflammatory changes after burn injury and most cytokine levels are increased on day 1 compared to on day 3 after burn injury. The concentrations of various cytokines in this study measured on day 1 or day 3 after burn injury failed to predict the prognosis related to patient’s survival or death.