P0194 Association between abdominal obesity and type 2 diabetes and HSP70-2 (+1267) gene mutation in colorectal cancer

Abstract

<h3>Background</h3> The number of patients with colorectal cancer (CRC) is increasing, as is mortality for patients with CRC. Previous reports have shown that heat-shock protein 70-2 (+1267) may be associated with CRC. However, genetic analysis has not been done for effect of the <i>HSP70-2</i> (+1267) gene in association with obesity and type 2 diabetes in CRC. The aim of this study is to investigate whether <i>HSP70–2</i> (+1267) gene polymorphism is involved in susceptibility to CRC in Iranian patients with obesity and type 2 diabetes and whether it correlates with CRC progression in these patients. <h3>Methods</h3> 50 genetically unrelated patients with CRC and 50 healthy controls were typed for the <i>HSP70-2</i> (+1267) PstI polymorphism with use of restriction fragment length polymorphism (RFLP) analysis (alleles A and B). Patients with CRC were classified into two types: those with obesity and type 2 diabetes and those without obesity and type 2 diabetes. <h3>Findings</h3> Our RFLP PCR analysis and polymorphism analysis revealed that <i>HSP70</i>-<i>2</i> has three type of allele, and that the BB allele (1267 A/G) was significantly more common in patients with obesity and type 2 diabetes than in those in the control group (<i>p</i>=0.0001). <h3>Interpretation</h3> Our data suggest that the BB allele of <i>HSP70-2</i> (+1267 A/G) in CRC patients is associated with hereditary obesity, and in lower efficiency, with type 2 diabetes.