Abstract
Optimum cryosurvival is dependent not only on freezing methodologies, but also on the type of cryopreservatives used. It seems reasonable to speculate that removing the seminal plasma and selecting highly motile spermatozoa by an optimal sperm processing technique, before freezing, may reduce the loss of viability, motility and acrosome integrity during cryopreservation. Moreover, this method can remove those cells that produce free radicals thereby minimizing peroxidative damage during freeze-thaw procedure.
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