Abstract
Purpose: Introduction: - Sickle cell anaemia also known as drepanocytosis, is a genetic blood related disease in which red blood cells form an cresent (sickle) or abnormal shape. This genetic disease is due to a change in the beta globin chain of haemoglobin glutamic acid (a polar amino acid) is replaced by valine (a non – polar) amino acid. This genetical change reduces the binding of haemoglobin for oxygen. At the low oxygen level the mutant haemoglobin, known as sickle haemoglobin (Hbss). Medicinal plants have been used in Indian folk medicine in the remedy of sickle cell anaemia by inhibiting sickling. This work was therefore aimed at screening the antisickling study of the aqueous extract of stems of T. Cardifolia and fruits of T.bellirica used in the Indian herbal medicine. Objective:- The present study investigates the preliminary screening of secondary metabolites of aqueous extracts of T. Cardifolia(stems) and T.bellirica(fruits) and invitro antisickling test (sickling reversal test). Materials and methods: Standard methods of phytochemical analysis of T. Cardifolia(stems) and T.bellirica(fruits) and sickling reversal test (invitro antisickling test), stems of T. Cardifolia,fruits of T.bellirica and Hbss blood samples etc. Results: Screening of secondary metabolites (bioactive molecules) and sickling reversal test were carried out using standard methods. The phyto-chemical screening indicated the presence of secondary metabolites (bioactive molecules) viz - alkaloids,flavonoids,phenols, saponins, steroids and cardiac glycosides etc. The results of antisickling reversal test that T. cardifolia(stems) and T. bellirica (fruits) exhibited potential antisickling property. Conclusion: The percentage of sickle cells of untreated Hbss was statistically significant higher than treated Hbss. The percentage of sickle cells decreased on the addition of aqueous extract of T. cardifolia stems and T. bellirica fruits on induced Hbss blood, which indicates the reversal of of Hbss blood to normal biconcave shape due to the presence of bioactive molecules, which have sickling reversal (anti-sickling property) and anti haemolytic properties. The authors do not declare any conflict of interest
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