Oxygen mass transfer and shear stress effects on Pseudomonas putida BCRC 14365 growth to improve bioreactor design and performance.

Abstract

In this work, the experimental evidence is presented for two basic issues including oxygen mass transfer and shear analysis on the microorganism containing medium on the most prominent sections of the bioreactor. Computational fluid dynamics (CFD) methodology reproduces shear rate values for specific impeller designs using the commercial code (Fluent 6.2). CFD calculates volumetric mass transfer coefficient based on the Higbie's penetration theory. Four types of impeller are used. The spherical probe is used to measure flow hydrodynamic parameters to obtain shear rate by electro-diffusion (ED) method. The obtained results are validated experimentally and it is shown that a fully axial pattern impeller represents more enhanced results than partially axial and radial. In this regard, experimental results for volumetric oxygen mass transfer coefficient (k l a) confirm CFD predictions by acceptable deviations of 2.65, 8.90, and 9.20 for 0.15, 0.2, and 0.3 VVM, respectively. These results collaboratively indicate that LIGHTNIN-C 200 type operates more efficiently by reflecting the flow to the bottom corner stagnation areas with the minimum tolerable shear and the most velocity distribution uniformity. Furthermore, the values of k l a improve by aeration rate. Conversely, increasing the rotational speed of impeller creates difficulties for cell growth due to the generated harsh shear condition. CFD provide a better understanding of how operational and geometrical variables may be manipulated to achieve a moderate shear rate and acceptable level of mass transfer.