Oxygen concentration of gut luminal contents varies post-prandially in growing pigs.

Abstract

The oxygen (O2 ) concentration of gastrointestinal tract (GIT) contents decreases distally, but little is known about how O2 concentrations are influenced by ingestion of a meal. The O2 concentration in luminal contents at different GIT locations (stomach [cardia and pylorus], proximal, mid- and distal small intestine and caecum) and how these concentrations changed post-prandially were determined. Fifty entire male pigs (22kg bodyweight at the start of study) were fed semi-synthetic diets containing casein, α-lactalbumin, whey protein isolate or zein as the sole source of protein for 8days. A further group of pigs received the casein diet for six days and a semi-synthetic protein-free diet for a further 2days. On day 8, pigs (n=2 per diet and time point) were euthanized post-prandially (0, 1, 2, 4 and 6h), and the stomach, small intestine and caecum were isolated and O2 determined in the GIT contents. Observations at each time point were averaged across the diets (n=10). The mean O2 concentration was markedly higher (p≤0.05) in the stomach compared with the rest of the GIT. The O2 concentration was similar in the small intestinal regions (p>0.05; 1.0%-1.1%) and the caecum (0.9%), apart for the proximal small intestine which had a 24% higher (p≤0.05) O2 concentration than the caecum. The mean O2 concentration in the GIT varied post-prandially (p≤0.05). The O2 concentration in the cardia decreased 1.8%/h over the first two hours post-feeding and thereafter increased 0.3%/h (p≤0.05). In the caecum, the O2 concentration was constant during the first 4h and thereafter increased slightly (p≤0.05). The flow of food through the GIT influenced both the concentration and amount of O2 in GIT luminal contents.