Abstract
The addition of sodium dihydrogen orthophosphate to storage buffers results in markedly higher intracellular ATP concentrations in rat skin stored at −196 and −3 °C as compared to storage in phosphate-free medium. The inorganic and total phosphorus depletion occurring in phosphatefree buffers can be compensated for by the addition of orthophosphates to the storage medium. The stimulatory effect of orthophosphate on the metabolic activity of stored tissue is attributed to an effective protection of oxidative phosphorylation. This is achieved by providing for the essential phosphate compounds necessary for constant resynthesis of ATP.
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