Abstract
The reaction mechanisms of oxidative degradation of the anaerobic radical enzyme pyruvate formate-lyase (PFL) have been examined at the theoretical hybrid Hartree−Fock/density functional theory level B3-LYP. It is concluded that the most likely scenario involves O2 addition at the glycyl radical site, followed by H• abstraction/transfer from C419 and subsequent rearrangements to generate an α-hydroxyglycine and the sulfinyl radical R−SO•, observed by EPR spectroscopy. In addition, the present model accounts for alternative fragmentations observed in wild-type PFL, and the formation and degradation of Gly−O-O• in the absence of C419 (as observed in C419A and C419AC418A mutants).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
