Abstract
We developed and used the nanocarbon film electrode to successfully detect epigenetic mark 5-hydroxymethylcytosine (5hmC) through direct oxidation after digesting genomic DNA (gDNA) with nuclease P1. The film showed superior key film properties including a very chemically stable structure and an ultraflat surface (Ra = 0.21 nm) that resulted in better electrode properties such as a wider potential window and lower background current than the widely used carbon electrode. Computational experiments revealed that the theoretical oxidation potential (Eox) value for 5hmC coincided with that obtained in electrochemical experiments using our nanocarbon film electrode. We successfully detected 5hmC by using this film electrode in combination with HPLC. Due to the difference of each oxidation potential, we achieved the oxidation potential-dependent selective detection of 5hmC in gDNA from excess amounts of unmethylated cytosine with a similar polarity to 5hmC.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
