Abstract
Methods are described to determine the effects of different substances on the oviposition behavior of Coleomegilla maculata lengi Timberlake. The preparation, bioassay, and chemistry of various materials from the wood of Juniperus virginiana L. are reported. Two preparations (A and B) acted as oviposition stimulants whereas surfaces treated with another fraction (C) of the extract were avoided. Materials A and B were high-molecular-weight polyphenols and showed general similarities to phlobaphenes from other sources. Material A caused apterous adult Acyrthosiphon pisum (Harris) to form aggregates on treated filter paper. O-Coumaric, salicylic, and protocatechuic acids, fluorescein, tannin, and widdrol at concentrations of 2.0–10.0 mg/50 cm2 influenced C. m. lengi to lay eggs on or near treated surfaces. Contact with fluorescein (2.0 mg/50 cm2) increased the proportion of laying C. m. lengi females.
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