Abstract
BackgroundPollen formation and development is important for crop fertility and is a key factor for hybrid development. Previous reports have indicated that Arabidopsis thaliana TAPETUM DETERMINANT1 (AtTPD1) and its rice (Oryza sativa) homolog, OsTPD1-like (OsTDL1A), are required for cell specialization and greatly affect pollen formation and development. Little is known about the role of the TPD1 homolog in banana pollen development.ResultsHere, we report the identification and characterization of TPD1 homologs in diploid banana (Musa itinerans) and examine their role in pollen development by overexpressing the closest homolog, MaTPD1A. MaTPD1A exhibits high expression in stamen and localizes in the plasma membrane. MaTPD1A-overexpressing plants produce no pollen grains and smaller and seedless fruit compared to wild-type plants. Transcriptome analysis showed that in plant hormone, starch and sucrose metabolism, and linolenic acid metabolism-related pathways were affected by overexpression of MaTPD1A, and the expression of several key regulators, such as PTC1 and MYB80, which are known to affect anther development, is affected in MaTPD1A-overexpressing lines.ConclusionsOur results indicate that MaTPD1A plays an important role in pollen formation and fruit development in diploid banana, possibly by affecting the expression of some key regulators of pollen development.
Highlights
Pollen formation and development is important for crop fertility and is a key factor for hybrid development
Determination and characterization of the Arabidopsis thaliana TAPETUM DETERMINANT1 (AtTPD1) homolog in the Banana a genome Using the Arabidopsis TAPETUM DETERMINANT1 (TPD1) (AAR25553) protein sequence as a query, a TBLASTN search was conducted against the banana A genome database
The blast search showed that MaTPD1A has conserved domains of AtTPD1 and shares 54% sequence identity and 75% sequence similarity with the Arabidopsis TPD1 protein (Fig. 1 and Table 1)
Summary
Pollen formation and development is important for crop fertility and is a key factor for hybrid development. Previous reports have indicated that Arabidopsis thaliana TAPETUM DETERMINANT1 (AtTPD1) and its rice (Oryza sativa) homolog, OsTPD1-like (OsTDL1A), are required for cell specialization and greatly affect pollen formation and development. In the anther of flowering plants, pollen formation results from the differentiation and interaction of reproductive cells (microsporocytes) with somatic anther wall cells (tapetum) [1]. The wild banana trees are diploid, fruits develop if pollinated, and are fully seeded [15]. The ovaries of female flowers develop into the fruit or finger, and clusters of banana fruit fingers are arranged into hands. In 1953, Fahn reported that in the Dwarf Cavendish banana plant the first flower is formed on the right side of the hand, and the remained flowers form in a zigzag pattern, back and forth between the top and the bottom rows [18]. Wild banana plants are diploid, generally cross-pollinated, and have fertile seeds. In the past thirty years, many banana-planting countries launched a series of crossbreeding programs and released many useful cultivars [21, 22]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
