Abstract
BackgroundThis study is conducted to investigate the effect of lncRNA PIK3CD-AS1 on the growth and metastasis of hepatocellular carcinoma (HCC) and its potential mechanism.MethodsHepatocellular carcinoma tissues and adjacent normal tissues together with HCC cells and normal liver cells were obtained for detecting expression of PIK3CD-AS1, microRNA-566 (miR-566) and LATS1. Additionally, a series of experiments were performed to determine cell proliferation, migration, invasion, cell cycle distribution and apoptosis of HCC cells. The xenograft tumor model of HCC was established and the growth rate and weight of xenograft tumor in nude mice were compared. Furthermore, the binding site between PIK3CD-AS1 and miR-566 as well as between miR-566 and LATS1 were verified.ResultsLncRNA PIK3CD-AS1 was downregulated in HCC tissues and cells, and mainly located in cytoplasm. Overexpression of PIK3CD-AS1 inhibited proliferation, colony formation, invasion, migration, epithelial–mesenchymal transition (EMT) and cell cycle progression and promoted apoptosis of HCC cells. Overexpression of PIK3CD-AS1 decreased the growth rate and weight of xenograft tumor in nude mice PIK3CD-AS1 competitively combined with miR-566 to regulate expression of LAST1.ConclusionCollectively, our study suggests that the expression of PIK3CD-AS1 was down-regulated in HCC, and overexpression of PIK3CD-AS1 promoted the expression of LATS1 by competitive binding of miR-566 to inhibit the growth, invasion and metastasis of HCC cells.
Highlights
This study is conducted to investigate the effect of lncRNA PIK3CD-AS1 on the growth and metastasis of hepatocellular carcinoma (HCC) and its potential mechanism
Expression of LncRNA PIK3CD‐AS1 is poor in HCC tissues and cells, and mainly located in cytoplasm The expression of PIK3CD-AS1 in HCC tissues and adjacent normal tissues was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR)
The results showed that the expression of PIK3CD-AS1 in HCC tissues was significantly lower than that in corresponding adjacent normal tissues (Fig. 1a; P < 0.01)
Summary
This study is conducted to investigate the effect of lncRNA PIK3CD-AS1 on the growth and metastasis of hepatocellular carcinoma (HCC) and its potential mechanism. A study has suggested that the expression of lncRNA PIK3CD-AS1 was significantly decreased in renal cell carcinoma (RCC) [12]. It is not completely clear whether the specific function of lncRNA PIK3CD-AS1 is implicated in the carcinogenesis of HCC. A previous study has revealed that miR-21 enables to resist radiation therapy by suppressing the expression of LATS1 in ovarian cancer cells [20], while its role in HCC is uncovered. We conducted this study to investigate the effect of lncRNA PIK3CD-AS1 on the growth and metastasis of HCC and its potential mechanisms
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