Abstract
Background: Diacylglycerol kinase iota (DGKI) is overexpressed in a variety of cancers and is associated with poor prognosis in colon cancer. This study evaluated the prognostic value of DGKI in gastric cancer (GC) using data from The Cancer Genome Atlas (TCGA).Methods: RNA sequencing results and clinical data of gastric adenoma and adenocarcinoma samples were obtained from the TCGA database (https://portal.gdc.cancer.gov). The Wilcoxon or Kruskal–Wallis test and logistic regression were used to analyze the relationship between DGKI and the clinicopathological characteristics of GC patients. Univariate Cox regression and Kaplan-Meier analysis were used to analyze the clinicopathological characteristics of GC patients and the relationship between DGKI and overall survival time, and multivariate Cox regression analysis was used to identify independent risk factors affecting the prognosis of GC patients. Gene set enrichment analysis (GSEA) was performed using the TCGA dataset.Results: DGKI was overexpressed in gastric tumors and was related to poor prognosis (p = 0.003). Overexpression of DGKI in GC was significantly correlated with high grade (OR = 1.71 for G3 vs. G2), stage (OR = 2.08 for II vs. I) and T classification (OR = 4.64 for T4 vs. T1; OR = 3.99 for T3 vs. T1; OR = 3.37 for T2 vs. T1) (all p <0.05). DGKI (OR = 7.34; p = 0.000) was an independent risk factor affecting the survival of GC patients. The MAPK signaling pathway was differentially enriched with DGKI overexpression.Conclusion: DGKI overexpression may be a potential molecular marker for poor prognosis in GC. The MAPK signaling pathway may be one of the key pathways related to DGKI regulation in GC.
Highlights
Gastric cancer (GC) is a common malignant tumor of the digestive system, and its mortality rate ranks third [1]
To identify the potential mechanism by which Diacylglycerol kinase iota (DGKI) expression affects the prognosis of gastric cancer patients, gene set enrichment analysis (GSEA) was used to first generate an ordered gene list based on the correlation between all genes and DGKI expression
We identified some genes that were related to the prognosis of GC patients and can be used as independent risk factors affecting the prognosis of GC patients in the data downloaded from the The Cancer Genome Atlas (TCGA) database (Supplementary Tables 1, 2)
Summary
Gastric cancer (GC) is a common malignant tumor of the digestive system, and its mortality rate ranks third [1]. Early identification and adjuvant chemotherapy are crucial for improving the survival of patients with advanced gastric cancer. Recent studies have found that mitochondrial dysfunction, signaling, fatty acid metabolism, and mitochondrial autophagy are related to DGKI Overexpression Predicts Poor Prognosis tumor growth [7, 8]. Studies [13] showed that DGKI was overexpressed in a variety of cancers and was associated with poor prognosis in colon cancer. The purpose of this study was to evaluate the prognostic value of DGKI expression in GC based on data obtained from The Cancer Genome Atlas (TCGA) and to conduct gene set enrichment analysis (GSEA) to identify the signaling pathways related to DGKI regulation in GC. Diacylglycerol kinase iota (DGKI) is overexpressed in a variety of cancers and is associated with poor prognosis in colon cancer. This study evaluated the prognostic value of DGKI in gastric cancer (GC) using data from The Cancer Genome Atlas (TCGA)
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