Abstract
Branched-chain α-keto acid dehydrogenase complex (BCKDC) is a multienzyme complex that plays an important role in branched-chain amino acid catabolism. It occupies a key position in intermediary metabolism and provides metabolic precursors for secondary metabolism. Multigenes encoding a bacterial BCKDC were introduced and overexpressed in Arabidopsis. The transgenic plants expressed an over seven-fold increase of BCKDC activity than control plants and accumulated significant high levels of branched-chain acyl-CoAs in seeds. Overexpression of the BCKDC also resulted in alternation of free amino acid composition in seeds with enhanced levels of branched-chain amino acids.
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