Abstract
BackgroundEndometriosis is a benign gynecological disease that shares some characteristics with malignant tumors and affects approximately 10% of women of reproductive age. Endometrioma refers to endometriosis that appears in the ovary. Metallopanstimulin-1 (MPS-1) is a component of the 40S subunit of ribosomes that has extra-ribosomal functions that contribute to the development of diseases. This study aimed to explore the expression pattern and role of MPS-1 in endometrioma development.MethodsQuantitative real time polymerase chain reaction, western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay were used to determine the expression of MPS-1 in patients with endometrioma. Following the successful knockdown of MPS-1 by siRNA, CCK-8 assays, flow cytometry, and transwell assays were performed to detect ectopic endometrial stromal cells (EcESCs) proliferation, the rate of apoptosis, and cell cycle, migration, and invasion, respectively. Western blotting was used to explore the effect of MPS-1 knockdown on protein levels in the NF-κB signaling pathway.ResultsThe expression of MPS-1 was significantly higher in endometrioma and the serum of endometrioma patients than in the patients without endometriosis. In addition, the downregulation of MPS-1 expression inhibited EcESCs proliferation, migration, and invasion. This downregulation led to the arrest of the EcESCs cycle in the G0/G1 phase and apoptosis and depressed the NF-κB signaling pathway.ConclusionMPS-1 can regulate EcESCs proliferation, motility, invasion, apoptosis, and cell cycle via the NF-κB signaling pathway in endometrioma. This may contribute to the formation or development of endometriotic foci. This study suggests the potential role of MPS-1 in the pathogenesis of endometriosis and enabled further research into the use of MPS-1 in the clinical diagnosis of endometrioma.
Highlights
Endometriosis, the presence of endometrial-like tissues outside the uterus cavity and myometrium, is a benign gynecological disease with characteristics resembling malignant tumors [1]
Ectopic tissues and paired eutopic endometrium were collected from 20 patients who were diagnosed with III/IV stage endometriosis with endometrioma according to the revised American Fertility Society scoring system
MPS‐1 is overexpressed in endometriosis patients Quantitative real time polymerase chain reaction (qRT-PCR) results showed that the mRNA levels of MPS-1 were significantly higher in endometrioma compared to both eutopic endometrium and control endometrium
Summary
Endometriosis, the presence of endometrial-like tissues outside the uterus cavity and myometrium, is a benign gynecological disease with characteristics resembling malignant tumors [1]. It affects approximately 10% of women of reproductive age, and up to 50% of endometriosis patients are infertile [2]. Endometrioma refers to endometriosis that appears in the ovary, taking up to 44% of endometriosis patients. Endometriosis is a benign gynecological disease that shares some characteristics with malignant tumors and affects approximately 10% of women of reproductive age. Endometrioma refers to endometriosis that appears in the ovary. This study aimed to explore the expression pattern and role of MPS-1 in endometrioma development
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