Otolith Stable Isotopes and Māori Archaeology

Model‐based sensitivity experiments are a widely used method for studying climate change attributions. In traditional climate sensitivity studies, “climate drift” occurs because of the accumulation of model errors during long‐term integrations, both in “actual” and “hypothetical” states. A more accurate piecewise‐integration method is used to reduce the model errors, dividing the continuous simulation into a series of sequential short‐term simulations. The model fields are updated at the end of each subinterval with analysis data for the “actual” state run, and with the sum of the analysis data and the difference between “hypothetical” and “actual” states for the “hypothetical” state run. This paper conducts sensitivity experiments with the continuous‐integration method and the piecewise‐integration method to evaluate the impacts of the central and eastern Pacific Ocean sea surface temperature anomalies (SSTAs) on the severe drought that occurred in southwestern China (SWC) in the winter of 2009/2010. The results show the following. (a) Model errors produced by the piecewise‐integration method for the actual state simulation are significantly less than those produced by the continuous‐integration method. (b) The intensity of the drought is significantly overestimated in continuous‐integration experiments. However, the drought can be accurately simulated in its spatial distribution and intensity via the piecewise‐integration method. Thus, climate sensitivity to changes in external forcing can be studied with greater credibility. (c) The warm central Pacific Ocean SSTAs may be the main cause of the drought over SWC. Adequate precipitation occurred over SWC when the centre of the SSTAs shifted to the east. (d) Warm SSTAs over the central Pacific Ocean influenced precipitation over SWC by weakening the water vapour transport branch from South China Sea to SWC. With a deficit of water vapour, the pronounced subsidence and warm temperatures were the main dynamic and thermodynamic factors that caused and maintained the drought.

Using observational data covering 1948–2020, the environmental factors controlling the winter precipitation in California were investigated. Empirical orthogonal function (EOF) analysis was applied to identify the dominant climate regimes contributing to the precipitation. The first EOF mode described a consistent change, with 70.1% variance contribution, and the second mode exhibited a south–east dipole change, with 11.7% contribution. For EOF1, the relationship was positive between PC1(principal component) and SST (sea surface temperature) in the central Pacific Ocean, while it was negative with SST in the southeast Indian Ocean. The Pacific–North America mode, induced by the positive SST and precipitation in the central Pacific Ocean, leads to California being occupied by southwesterlies, which would transport warm and wet flow from the ocean, beneficial for precipitation. As for the negative relationship, California is controlled by biotrophically high pressure, representing part of the Rossby wave train induced by the positive SST in the Indian ocean, which is unfavorable for the precipitation. For EOF2, California is controlled by positive vorticity at the upper level, whereas at the lower level, there is positive vorticity to the south and negative vorticity to the north, the combination of which leads to the dipole mode change in the precipitation.

Migration routes and the depth patterns of anguillid eel larvae migrating long distances from spawning grounds in the ocean remain poorly understood. We used otolith stable isotope analysis to study the oceanic migrations of anguillid eels by reconstructing experienced water temperature histories of larvae. The otolith stable oxygen isotopes (δ18Ootolith) of recruited Anguilla japonica glass eels were analyzed to assess the relationship with the experienced water temperature of the early larval stage in laboratory experiments. A negative linear relationship between rearing water temperature and δ18Ootolith values was observed for eel leptocephali reared at five different temperatures between 19 °C and 27 °C. Subsequently, the obtained equation between water temperature and δ18Ootolith was applied to estimate the water temperature experienced by wild-caught glass eels during their larval oceanic migration according to recruitment latitude, season, and species. The δ18Ootolith values of A. japonica glass eels were significantly higher at higher recruitment latitudes from Taiwan to Japan and later recruitment periods of 6 months, from November to March, indicating that the individuals were exposed to lower water temperatures along the Kuroshio flowing northward as they reached the higher latitudes in the later recruitment seasons. Furthermore, the δ18Ootolith values of the temperate A. japonica were higher than those of the tropical Anguilla marmorata, recruited at the same locations in southern Japan and Taiwan. This suggests that larval migratory behavior in the ocean may differ between the two eel species, although they have sympatric spawning areas in the western North Pacific. Collectively, these results suggest that δ18Ootolith provides a reasonable estimation of the experienced water temperature and may prove useful for reconstructing the early migratory history of anguillid eels in the ocean.

Stable carbon isotope analysis has proven utility for reconstructing dietary information in humans in past populations. The usefulness of stable carbon for forensic geolocation has been little investigated, largely because of the globalization of the human diet seemingly rendering it inconsequential. This study queried this assumption at a country-wide level on a known sample group. Stable carbon isotope values were obtained from human enamel with known biographical and geographical information to determine whether stable carbon, when paired with stable oxygen isotope values, could differentiate Canadians from non-Canadians. Samples originating outside of Canada were separated into three regions, and a linear discriminant analysis was used to generate discriminant functions that best separate the regions according to the stable carbon and oxygen isotope values. The results revealed two functions, where the first function explained 92.1% and the second 7.9% of the variance. Although some overlap in stable carbon and oxygen values was observed for individuals from both the United States and Canada, differences were observed between those from Canada and other geographical regions. This study demonstrated that pairing the dietary isotope carbon with the geolocator stable oxygen isotope, produced an interesting separation geographically, one that might well be helpful when attempting a geolocation query on unknown human remains.

SummaryTo quantify the major environmental drivers of stream bacterial population dynamics, we modelled temporal differences in stream bacterial communities to quantify community shifts, including those relating to cyclical seasonal variation and more sporadic bloom events. We applied Illumina MiSeq 16S rRNA bacterial gene sequencing of 892 stream biofilm samples, collected monthly for 36‐months from six streams. The streams were located a maximum of 118 km apart and drained three different catchment types (forest, urban and rural land uses). We identified repeatable seasonal patterns among bacterial taxa, allowing their separation into three ecological groupings, those following linear, bloom/trough and repeated, seasonal trends. Various physicochemical parameters (light, water and air temperature, pH, dissolved oxygen, nutrients) were linked to temporal community changes. Our models indicate that bloom events and seasonal episodes modify biofilm bacterial populations, suggesting that distinct microbial taxa thrive during these events including non‐cyanobacterial community members. These models could aid in determining how temporal environmental changes affect community assembly and guide the selection of appropriate statistical models to capture future community responses to environmental change.

The Atlantic mackerel (Scomber scombrus) in the Northwest Atlantic is comprised of northern and southern components that have distinct spawning sites off Canada (northern contingent) and the US (southern contingent), and seasonally overlap in US fished regions. Thus, assessment and management of this population can be sensitive to levels of mixing between contingents, which remain unknown. Multi-decadal trends in contingent mixing levels within the US fisheries region were assessed, and the contingent composition across seasons, locations, ages, and size classes were characterized using archived otoliths and developing a classification baseline based on juvenile otolith carbon and oxygen stable isotopes (δ13C/δ18O values). Classification of age ≥ 2 adults demonstrated that northern contingent mixing was prevalent within the US continental shelf waters during the past 2 decades (2000–2019), providing an important seasonal subsidy to the US winter fishery despite substantial depletion in spawning stock biomass of the dominant northern contingent. While the majority of older fish were of the northern contingent during the early 2000s, the southern contingent contribution increased with age/size class during the recent period (2013–2019). Spatial mixing was most prevalent during February and March when the northern contingent occurred as far south as the Delmarva Peninsula, but were mostly absent from US waters in May. A positive relationship (albeit not significant; r = 0.60, p = 0.07) occurred between northern contingent mixing and US fisheries landings, which could imply that higher contingent mixing levels might be associated with greater landings for the US winter mackerel fishery. The yield of the Northwest Atlantic mackerel depends upon the status of the northern contingent, with the southern contingent possibly more prone to depletion. Spatially explicit stock assessment models are recommended to conserve both productivity and stability in this two-component population.

The dynamic origin of the interannual variability of West China autumn rainfall (WCAR), a special weather/climate phenomenon over western-central China in September and October, was investigated via observational diagnosis and numerical simulations. Here we found that the interannual variability of WCAR is closely related to the local horizontal trough, which is passively induced by two lower-level anticyclonic (high pressure) anomalies over East Asia. The anticyclonic anomaly over the south is a Gill-type response to the central and eastern Pacific diabatic cooling, while that over the north is part of the mid- to high-latitude barotropic Rossby wave train, which could be induced by either the thermal forcing of the central and eastern Pacific Ocean sea surface temperature (SST) cooling or that of the subtropical northern Atlantic Ocean SST warming. The quasi-barotropic high pressure anomaly over East Asia acts as an “invisible mountain” that steers the low-level anomalous southwesterly into a southeasterly and hinders the water vapor going farther to the north, leading to enhanced WCAR. However, the real mountain ranges in the region (the Qinglin and Ba Mountains) have no essential impact on the formation and interannual variability of WCAR.

– Stable oxygen isotopes (δ18O) derived from otoliths were used to estimate mean annual water temperatures experienced by individual Svalbard Arctic charr, Salvelinus alpinus (L.), during their first four growth seasons. The analysed Arctic charr experienced a high variety of temperatures, indicating the use of different thermal habitats. A higher proportion of the juveniles experienced warmer temperatures during their first summer compared with later summers, suggesting the selective use of the shallowest littoral areas of the lake. Although the estimated temperatures were consistent with water temperatures found in High Arctic rivers and lakes during summer, they did not represent the annual variation in air temperature registered over the 20 years of otolith measurement. Furthermore, summer otolith increment width did not correlate with the experienced temperature. However, after the second year, otolith increment width was highly dependent on increment width during the previous summer. This study estimated mean summer water temperatures experienced by individual Arctic charr during the first four growth seasons providing additional evidence that stable oxygen isotope analysis can be used to provide insight into the thermal habitat use by juvenile Arctic charr.

Physoclist fish are able to regulate their buoyancy by secreting gas into their hydrostatic organ, the swim bladder, as they descend through the water column and by resorbing gas from their swim bladder as they ascend. Physoclists are restricted in their vertical movements due to increases in swim bladder gas volume that occur as a result of a reduction in hydrostatic pressure, causing fish to become positively buoyant and risking swim bladder rupture. Buoyancy control, rates of swim bladder gas exchange and restrictions to vertical movements are little understood in marine teleosts. We used custom-built hyperbaric chambers and laboratory experiments to examine these aspects of physiology for two important fishing target species in southern Australia, pink snapper (Pagrus auratus) and mulloway (Argyrosomus japonicus). The swim bladders of pink snapper and mulloway averaged 4.2 and 4.9 % of their total body volumes, respectively. The density of pink snapper was not significantly different to the density of seawater (1.026 g/ml), whereas mulloway were significantly denser than seawater. Pink snapper secreted gas into their swim bladders at a rate of 0.027 ± 0.005 ml/kg/min (mean ± SE), almost 4 times faster than mulloway (0.007 ± 0.001 ml/kg/min). Rates of swim bladder gas resorption were 11 and 6 times faster than the rates of gas secretion for pink snapper and mulloway, respectively. Pink snapper resorbed swim bladder gas at a rate of 0.309 ± 0.069 ml/kg/min, 7 times faster than mulloway (0.044 ± 0.009 ml/kg/min). Rates of gas exchange were not affected by water pressure or water temperature over the ranges examined in either species. Pink snapper were able to acclimate to changes in hydrostatic pressure reasonably quickly when compared to other marine teleosts, taking approximately 27 h to refill their swim bladders from empty. Mulloway were able to acclimate at a much slower rate, taking approximately 99 h to refill their swim bladders. We estimated that the swim bladders of pink snapper and mulloway ruptured after decreases in ~2.5 and 2.75 times the hydrostatic pressure to which the fish were acclimated, respectively. Differences in buoyancy, gas exchange rates, limitations to vertical movements and acclimation times between the two species are discussed in terms of their differing behaviour and ecology.

The diets of eight demersal fish species from the upper continental slope (c. 24CM‐50 m) were determined from samples taken during late January and early February 2004, off the Wairarapa coast, North Island, New Zealand. Diets were from a combination of benthopelagic and benthic sources, with most species exhibiting ontogenetic shifts in diet, in that larger‐bodied food (fish and/or natant decapod prawns) was more important in the diets of larger fish. Javelinfish (Lepidorhynchus denticulatus), silver roughy (Hoplostethus mediterraneus), and capro dory (Capromimus abbreviatus) had predominantly benthopelagic diets, whereas the diets of sea perch (Helicolenus percoides), Bollons’ rattail (Caelorinchus bollonsi), two‐banded rattail (C. biclinozonalis), and silverside (Argentina elongata) were predominantly benthic, with the crab Carcinoplax victoriensis an important food item. The diet of Oliver's rattail (C. oliverianus) was a mix of benthopelagic and benthic organisms. Generally, levels of dietary overlap between the eight species were low. Overall, the diets of these fish probably reflect regional and seasonal levels of food availability. Notes on food observed in the stomachs of a further 18 species, for which there were fewer than 10 stomach samples per species are also provided.

Mangroves and seagrass beds have long been perceived as important nurseries for many fish species. While there is growing evidence from the Western Atlantic that mangrove habitats are intricately connected to coral reefs through ontogenetic fish migrations, there is an ongoing debate of the value of these coastal ecosystems in the Indo-Pacific. The present study used natural tags, viz. otolith stable carbon and oxygen isotopes, to investigate for the first time the degree to which multiple tropical juvenile habitats subsidize coral reef fish populations in the Indo Pacific (Tanzania). Otoliths of three reef fish species (Lethrinus harak, L. lentjan and Lutjanus fulviflamma) were collected in mangrove, seagrass and coral reef habitats and analyzed for stable isotope ratios in the juvenile and adult otolith zones. δ13C signatures were significantly depleted in the juvenile compared to the adult zones, indicative of different habitat use through ontogeny. Maximum likelihood analysis identified that 82% of adult reef L. harak had resided in either mangrove (29%) or seagrass (53%) or reef (18%) habitats as juveniles. Of adult L. fulviflamma caught from offshore reefs, 99% had passed through mangroves habitats as juveniles. In contrast, L. lentjan adults originated predominantly from coral reefs (65–72%) as opposed to inshore vegetated habitats (28–35%). This study presents conclusive evidence for a nursery role of Indo-Pacific mangrove habitats for reef fish populations. It shows that intertidal habitats that are only temporarily available can form an important juvenile habitat for some species, and that reef fish populations are often replenished by multiple coastal habitats. Maintaining connectivity between inshore vegetated habitats and coral reefs, and conserving habitat mosaics rather than single nursery habitats, is a major priority for the sustainability of various Indo Pacific fish populations.

Glacial-interglacial changes in global and regional temperature have been linked to changes in insolation, winds, and ocean circulation. This study reconstructs sea surface temperature (SST) and source water provenance across the last deglaciation (~30-5 kyr BP) in five sediment cores in the Bay of Plenty, New Zealand and places these in a regional context by comparing these results to previously published work.SST reconstructions from Mg/Ca ratios in the planktonic foraminiferaGlobogerina bulloides and alkenones (Ú) track different seasons’ SST spring and summer, respectively. During the last glacial maximum (LGM, 26-22 kyr BP), summer SSTs average 16.4°C while spring SSTs were 13.6; about 4°C cooler than modern. The seasons track well with each other and maintain a constant offset of 3.3-2.8°C as temperatures increase into the Holocene, peaking at 21.7°C for summer and 18.4°C for spring. Comparison to model reconstructions of local insolation yielded correlation to winter insolation from the LGM (~26 kyr BP) to the Antarctic Cold Reversal ACR (~14.1 kyr BP) after which SSTs correlate well to their respective seasonal insolation. Comparison of this study’s temperatures to published SSTs indicate that deglacial warming of subtropical waters differ from subantarctic waters that warmed later and by 2°C more than subtropical waters.δ18O and δ13C from planktonic foraminifera G. bulloides and Globorotalia inflate were used to reconstruct δ18O of seawater (δ18Osw) and track source water provenance. In the LGM, depleted δ18OSW averaging 0.2‰, and enriched δ13C ranging between -0.4-0.1‰ indicate shallow water masses had a strong local Southern Ocean component. A step change occurs at 20.1 kyr BP where δ13C depletes to -1.3‰ that suggests a deglacial shift in shallow subsurface water mass source location to a distal subtropical component likely sourcing through the equatorial Pacific that persists into the Holocene. A regional comparison indicates numerous switches between distal-subtropical and proximal- subantarctic influences during the early deglaciation. This ends at the ACR, which figures as a tipping point for stabilization and onset of modern circulation.

Stable Carbon and Oxygen Isotopes in Recent Sediments of Lake Wigry, NE Poland: Implications for Lake Morphometry and Environmental Changes

Stable isotope stratigraphy of Holocene speleothems: examples from a cave system in Rana, northern Norway

Abstract. Capturing the mechanisms leading to the local extirpation of a species in deep time is a challenge. Here, by combining stable oxygen and carbon isotopic analyses on benthic and planktonic foraminifera and the otoliths of pelagic and benthic fish species, we reveal the paleoceanographic regime shifts that took place in the eastern Mediterranean from 7.2 to 6.5 Ma, in the precursor phase to the Messinian salinity crisis, and discuss the fish response to these events. The stepwise restriction of the Mediterranean–Atlantic gateway impacted the metabolism of fishes in the Mediterranean, particularly those dwelling in the lower, deeper part of the water column. An important shift in the Mediterranean paleoceanographic conditions took place between 6.951 and 6.882 Ma, from predominantly temperature to salinity control, which was probably related to stratification of the water column. A regime shift at 6.814 Ma, due to changes in the influx, source and/or preservation of organic matter, led to pelagic–benthic decoupling of the fish fauna. At that time, the oxygen isotopic composition of benthic fish otoliths reflects higher salinity in the lower part of the water column that is accompanied by a rapid fluctuation in the carbon isotopic composition (a proxy for the metabolic rate), ultimately leading to the local extirpation of the benthic species. Overall, our results confirm that otolith stable oxygen and carbon isotope ratios are reliable proxies for paleoceanographic studies and, when combined with those of foraminifera, can reveal life history changes and migration patterns of teleost fishes in deep time.