Abstract
Mouse renal transplantation is a technically challenging procedure. Although the first kidney transplants in mice were performed over 34 years ago and refined some years later, the classical techniques of mouse renal transplantation required clamping both vena cava and aorta simultaneously and carry out suture anastomoses of the renal artery and vein in a heterotopic position. In our laboratory, we have successfully developed mouse orthotopic kidney transplantation for the first time, using a rapid “cuffed” renal vein technique for vessel anastomosis, wherein the donor’s renal vein was inserted through an intravenous catheter, folded back and tied. During grafting, the cuffed renal vein was directly inserted into the recipient’s renal vein without the need for the clamping vena cava and suturing of renal vein. This technique allowed for the exact transplantation of the kidney into the original position, compared to the classical technique, and has significantly shortened the clamping time due to a quicker and precise anastomosis of renal vein as described. This also allowed for a quicker recovery of the lower extremity activity, reduction in myoglobinuria with resultant kidney graft survival of 88.9%. Thus we believe that the cuffed renal vein technique simplifies microvascular anastomoses and affords important additional benefits.
Highlights
Murine kidney transplantation has gained widespread use as genetically modified strains can be used to study the molecular mechanisms of renal allograft injury [1,2]
The cuffed renal vein technique significantly shortened the operation time Overall operative time, aortic cross clamp time and lower legs activity recovery time were compared between the classical technique (n=20) and the new cuffed renal vein technique (n = 30)
The overall operative time was reduced with the new cuffed renal vein technique, as evidenced by the significantly reduced times required to prepare the renal anastomoses (p
Summary
Murine kidney transplantation has gained widespread use as genetically modified strains can be used to study the molecular mechanisms of renal allograft injury [1,2]. Skoskiewicz et al reported the first mouse kidney transplantation in 1973 [3], which was later improved by Zhang and Han [4,5], and recently modified by Song Rong [6] This classical technique required cross-clamping proximally and distally a section of aorta and inferior vena cava (IVC) with two microvascular clamps, elliptical arteriotomy and longitudinal venotomy , end-to-side anastomosis of the donor renal vessels to the aorta and IVC below the level of the native renal vessels. This technique has the disadvantage of aortic and vena caval cross clamping which prolongs the ischemia time of lower extremities and subsequently inducing rhabdomyolysis and worsening renal function. Long-term survival and renal function with the new technique were monitored over 10 weeks
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