Abstract
Abstract Application of rhizobial inoculant is an agricultural practice successfully used to increase growth and yield of leguminous plants. Development of a concentrated culture is essential for advanced formulations. This study assessed the use of starch industry wastewater as a potent carbon source for production of Sinorhizobium meliloti, with a maximum cell count of 4×109 CFU/mL. Optimal conditions for maximum recovery of cells (>99%) during centrifugation were determined to be 8 000 g, at a temperature of 20 °C for 20 min, pH 7, using a swinging bucket rotor and a surface response methodology. Of the different centrifugal aids tested, starch (2% w/v) best minimized the loss of microbial cells during recovery from the supernatant. A fixed- angle-rotor experiment was also carried out to determine differences in recovery between centrifugal configurations; optimal recovery was observed with the swinging bucket rotor (>99%) versus a fixed angle rotor (90%). A further decrease (from 90% to 87%) in recove...
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