Abstract

This study was conducted to investigate essential oil based genetical and physiological changes on maize seedling under laboratory conditions. The aerial parts of O. rotundifolium BOISS. Were powdered, and EO obtained by hydro-distillation. For the best results, the content applied to Gas Chromatography/ Mass Spectrometry (GC-MS), and the main constituents have been determined as Carvacrol (%43.62) and Thymol (%40.86). The liquid phase of the oil applied to maize seeds at four concentrations. Randomly Amplified Polymorphic DNA method was used to observe possible DNA alterations. With the total soluble protein level and SDS Page analysis, the physiological effects of EO were determined. The oils showed phytotoxic effects, but not in a dose-dependent manner. Soluble protein level and SDS Page analysis did not show any significant change but genomic template stability value was decreased with the increasing of EO dose.

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