Abstract
Polarization-resolved second-harmonic-generation (PR-SHG) microscopy simultaneously visualizes the orientation angle and degree of crystallinity in collagen fibers in tissues. In this paper, we developed fast PR-SHG microscopy based on pixel-by-pixel rapid polarization switching applicable for in situ measurements of the human skin. The demonstrated results indicated that our system would be a powerful tool for optical assessment of dermal collagen fiber in cosmetics research.
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