Organogénesis in-vitro using three tissues types of tree tomato [ Solanum betaceum (Cav.)

Abstract

Tree tomato is a fruit with great economic potential due to its high nutritional value. The induction of direct organogenesis in this species is a great alternative for clonal propagation of disease-free plants, and also useful for genetic transformation. In this study was assessed the induction of organogenesis in vitro using three different tissues: leaves, petioles and sexual seeds of the variety Common. All tissues were cultured on MS supplemented with agar, sucrose and TDZ or BAP phytohormones at 0.5 to 3 mg L-1, combined or not with the auxins IAA and NAA. Although petioles and seeds had a great potential for regeneration of plantlets, leaves produced more shoots (average of 18.4 shoots/explant) on medium containing TDZ 0.5 mg L-1. Additionally, its easy handling and resistance to physical damage may be useful traits to perform genetic trans-formation. The highest average of shoots produced from seeds and petioles were 4.3 and 3.1 shoots/explant, respectively. The production of multiple shoots in petioles was influenced by low concentrations of auxins. The emergence of the radicle in the seeds was important for the formation of shoots.