Abstract

Genetic modification has great advantages in improving performance of bacteria, but its oral safety has not been systematically evaluated. In this study, the toxicity including the reproductive toxicity of two genetically modified bacteria engineered using food-grade vectors on three generations of rats (F0, F1 and F2) were studied. Sprague Dawley rats were administrated by gavage with corresponding parent and genetically modified Lactobacillus plantarum and Lactobacillus delbrueckii at 2.5 × 10 9 CFU every other day for 8 weeks. Results showed that the transgenic Lactobacillus had no significant toxicity to the body weight, food intake or blood biochemical parameters of three generations of rats. There was no significant effect on the reproductive parameters (gestational weight and pregnancy time) and related hormones (FSH, LH, estradiol, progesterone and testosterone) of the parent rats (F0 and F1). Consistently, their offsprings had no abnormal physical conditions including body weight, body length and anogenital distances. Moreover, their pups had normal body organ weight and reasonable abnormal sperm rate. Further analyses were conducted to evaluate SOD, MDA and GPX of the ovarium in the F1 and F2 female rats, which showed no malady phenotypes. In conclusion, transgenic Lactobacillus has no toxicity to the three generations of rats.

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