Optimum Combination of Radiopharmaceuticals‐Based Targeting‐Triggering‐Therapy Effect and PD‐L1 Blockade Immunotherapy

Abstract

AbstractThe therapeutic alliance of 177Lu radioligand therapy and immune checkpoint blockade (ICB) has gained increasing attention. This study aims to investigate the immunomodulatory effect (targeting‐triggering‐therapy) of 177Lu‐DOTA‐EB‐cRGDfK (177Lu‐DER) and to optimize the therapeutic efficacy by combining targeted radionuclide therapy (TRT) and ICB. Flow cytometry, immunofluorescence analysis, and RT‐qPCR are conducted to confirm the change of PD‐L1 expression. Tumor uptakes of 177Lu‐DER in CT26 and MC38 colorectal tumor models are verified through single photon emission computed tomography imaging. Further, the radionuclide dose and the treatment schedule to optimize the therapeutic scheme are carefully titrated and the mechanism of the synergy between TRT and ICB is explored. The results demonstrate that PD‐L1 expression is upregulated after irradiation of 177Lu. The combination of 9.25 MBq 177Lu‐DER TRT with 200 µg αPD‐L1 immunotherapy significantly inhibits tumor growth and protects against tumor recurrence. It is also found that 4‐h interval is an effective time window between radioligand administration and ICB therapy. In conclusion, a promising scheme for tumor immunotherapy is realized based on the sequential administration of integrin αvβ3‐targeted radioligand and PD‐L1 immune checkpoint blocker, emphasizing the potential of combining radiotheranostics with ICB for precision cancer therapy.