Abstract
Wind Cave near Hot Springs, South Dakota has over 150 miles of surveyed area and is yet to be completely mapped. Identifying the types of microorganisms living in the cave will provide insights into understanding what types of organisms could be found in other extreme environments. The main question to be answered in this work is which commercial DNA isolation kits provide the best yield of purified DNA from microorganisms collected at Wind Cave. Samples from the cave were collected and grown on culture media. Taking an unbiased approach, seven commercially available DNA isolation kits were evaluated using the manufacturers’ recommended protocols. Kits from Omega Bio-tek and Qiagen exhibited optimal performance and were most effective at isolating DNA from the samples collected in Wind Cave. The DNA obtained from the samples will be used to perform PCR-based sequencing analysis using primers targeting either a specific region of the 16S small ribosomal subunit of prokaryotes for bacteria and archaea or an internal transcribed spacer (ITS) for identifying fungal microbial eukaryotic lineages.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
