Abstract

Global warming, urban pollution and depletion of fossil fuels have been driving for looking alternative energy sources, especially those derived from biomass. Production of bio-ethanol from lingocellulosic materials is providing a long-term sustainable for fuel supply. <i>Striga hermonthica</i>, a parasitic weed plant is one of cheap source of lignocellosic materials to serve as feedstock for bio-ethanol production. With the objective of evaluating its potential for bio-ethanol production, different concentrations (10g, 20g, 30g, and 40g) of <i>Striga hermonthica</i> treated with 1% diluted sulfuric acid and untreated were subjected to batch fermentation for 16 days with 0.5% and 1% yeast inoculums. Percent of bio-ethanol production, cell density and reducing sugars were measured at an interval of 4 days starting from the beginning. Results of these study showed that ethanol production was observed starting from the 4<sup>th</sup> day of fermentation, but its amount peaked 28.05% from 40g substrate with 1% inoculum on the 12<sup>th</sup> day of fermentation, and declined on 16<sup>th</sup> days (20.24%) from the same substrate concentration. Pretreated substrate showed significantly higher ethanol production than untreated. In agreement with ethanol production, cell density and reduction in reducing sugar were observed in the same pattern. Compared ethanol production between untreated substrates yield of 21.31%, and treated substrates yielded of 28.05%. Overall, this study showed that acid pre-treatment, inoculum concentration, fermentation period and substrate concentration affect the amount of bio-ethanol production. Finally, it can be concluded that the production of bioethanol from <i>Striga hermonthica</i> is economically and environmentally viable. Extensive use of this harmful weed for bioethanol production may have twofold advantages, viz. reduction of its negative impact on crop productivity and generation of bio-ethanol.

Highlights

  • Bio-ethanol is one of the liquid fuels found to have an efficient calorific content capable of being used in automobiles [15]. It is derived from renewable feedstock sources such as sugary, starchy and lignocellulosic biomass

  • The production of bio-ethanol from comparatively cheaper source of raw materials using efficient fermentative microorganisms is the only possible way to meet the great demand for bio-ethanol in the present situation of energy crisis. Alternative biomass sources such as agricultural and municipal wastes, and other lignocellulosic materials from non-food plants being sought as alternatives source [11]

  • Temam Gemeda Genemo: Optimizing Bio-ethanol Production from Striga hermonthica Using Yeast (Saccharomyces cerevisiae) as a Fermenting Agent were observed and results were indicated in table 1

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Summary

Introduction

Bio-ethanol is one of the liquid fuels found to have an efficient calorific content capable of being used in automobiles [15]. It is derived from renewable feedstock sources such as sugary, starchy and lignocellulosic biomass. In Ethiopia, especially eastern region, grain yield loss for susceptible sorghum varieties was estimated to be 59% due to the infection of Striga weed species [2] Extensive use of this harmful weed for bioethanol production may have twofold advantages, viz. General objective of the study To determine the amount of bio-ethanol production from Striga hermonthica by a batch culture using S. cerevisiae. Specific objectives of the study are 1) To identify the optimum substrate concentration for maximum bio ethanol production 2) To determine the cell density and reducing sugar concentrations at different fermentation time 3) To assess the effect of acid pre-treatment on the rate of ethanol production

Fermentation Processes
Estimation of Total Reducing Sugar
Substrate Preparation
Inoculums Preparation
Amounts of Ethanol Production from Fermenter
Amounts Reducing Sugar Yield
Cell Density Concentrations at Different Fermentation Period
F Untreated G Untreated H Untreated
Summary
Conclusion
Recommendation
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