Abstract
Thermo-alkali-stable xylanase producing Bacillus tequilensis strain UD-3 was isolated from the hot spring, and the biobleaching effect on rice straw pulp was evaluated. Bacillus tequilensis strain UD-3 produced xylanase (8.54 IU mL−1) employing on wheat bran. The xylanase production was increased about 2 fold after optimization of media via central composite design. The maximum xylanase activity was achieved at 8.0 pH, 50 °C temperature, and boosted at 1.0 mM concentration of metal ions such as Mn+2, Fe+3, Cu+4, and Zn+4. Furthermore, Zn+4 concoction with xylanase was evaluated for pre-bleaching of rice straw pulp in which xylanase significantly reduce the Kappa number (17.24 ± 0.02) after Xyl + Zn bleaching. A significant reduction was also observed in the reducing sugars (50%), phenolic (29.19%), hydrophobic (33.20%), and lignin compounds (35.86 and 40.48%) during the Xyl + Zn treatment of pulp samples in comparison to the only xylanase.
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