Abstract
Phenolic compounds were extracted from the husk of milled black rice (cv. Poireton) by using a combination of ultrasound assisted extraction and microwave assisted extraction. Extraction parameters were optimized by response surface methodology according to a three levels, five variables Box-Behnken design. The appropriate process variables (extraction temperature and extraction time) to maximize the ethanolic extraction of total phenolic compounds, flavonoids, anthocyanins and antioxidant activity of the extracts were obtained. Extraction of functional components with varying ethanol concentration and microwave time were significantly affected by the process variables. The best possible conditions obtained by RSM for all the factors included 10.02min sonication time, 49.46°C sonication temperature, 1:40.79 (w/v) solute solvent ratio, 67.34% ethanol concentration, and 31.11s microwave time. Under the given solutions, the maximum extraction of phenolics (1.65mg/g GAE), flavonoids (3.04mg/100g), anthocyanins (3.39mg/100g) and antioxidants (100%) were predicted, while the experimental values included 1.72mg/g GAE of total phenolics, 3.01mg/100g of flavonoids, 3.36mg/100g of anthocyanins and 100% antioxidant activity. The overall results indicated positive impact of co-application of microwave and ultrasound assisted extractions of phenolic compounds from black rice husk.
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