Abstract
Steam explosion pretreatment was conducted on seabuckthom pomace. Response surface methodology was used to optimize the treatment conditions of steam explosion, including steam pressure, duration and particle size. After this, the content of total flavonoids and the antioxidant capacity of total flavonoids were investigated. Results showed that when the steam pressure was 2.0 MPa, duration was 88 s and a sieving mesh size was 60, the total flavonoids content in seabuckthorm reached a maximum of 24.74 ± 0.71 mg CAE/g, an increase of 246% compared with that without steam explosion treatment (7.14 ± 0.42 mg CAE/g). Also, DPPH and ·OH free radical scavenging ability showed significant improvement, with an IC50 decrease to 13.53 μg/mL and 4.32 μg/mL, respectively, far lower than that in original samples. Through the scanning electron microscope, the surface of seabuckthom pomace after steam explosion was crinkled, curly, and holey. Our study showed that the content of total flavonoids in seabuckthom pomace could be obviously promoted and the antioxidant capacity of total flavonoids also improved significantly, after applying steam explosion pretreatment to seabuckthom pomace, making this approach meaningful for the reuse of seabuckthom pomace resources.
Highlights
Seabuckthom, a perennial deciduous shrub or small arbor, is a kind of small berry plant that favors sunlight, resists water and drought, and enjoys vigorous growth
Quadratic linear regression fitting and significance and variance analysis are applied to the data, and a second-order polynomial equation is given to express the dependent variable and response value
The results shown that the yield of total flavonoids (TFs) increased by 246% after steam explosion, proving that optimization of steam explosion pretreatment conditions for Seabuckthom pomace (SP) by the response surface methodology is feasible
Summary
Seabuckthom, a perennial deciduous shrub or small arbor, is a kind of small berry plant that favors sunlight, resists water and drought, and enjoys vigorous growth. The widespread planting of seabuckthom can prevent wind, fix sand, maintain water and soil, and facilitate the protection of the ecological environment, and more importantly, it possesses the characteristic of being useful as a medicine and food owing to its rich content of nutrients like vitamins, sugar, proteins, amino acids and microelements, as well as biological active substrates. It has attracted widespread attention in the research and development of healthcare products and foods [2,3,4]
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