Abstract
In the present investigation natural phenolic antioxidants were extracted from Jojoba seed waste. Samples of defatted jojoba seed meal (JSM), detoxified meal residue (DMR) and jojoba seed shell (JSH) were subjected to extraction by using various solvents: 70/30 (vol/vol) ethanol/water, 70/30 (vol/vol) methanol/water and 60/40 (vol/vol) acetone/water. Total phenolics content (TPC) is measured according to the Folin-Ciocalteu method. In addition, solvent extracts were tested for their antioxidant activity (AA) in vitro by the DPPH radical scavenging and the β-carotene linoleic acid bleaching methods. The highest amount of total phenolic compounds (TPC) is observed with 60% acetone extract of JSH (163.81±6.88 mg GAE g -1 extract DW). On the other hand, the 70% Methanolic extract of JSH indicates the highest activity in scavenging DPPH free radicals. DMR extracts had low amount of phenolics. Furthermore, the ethanolic extract of JSM and JSH at concentration 200ppm showed good antioxidant capacity for stabilization of super palm olein (SPO) than BHT as determined by the rancimat procedure. Therefore, these extracts could be a good alternative for synthetic antioxidant to protect the vegetable oils such as SPO which contain different levels of unsaturated fatty acids.
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