Abstract
The subcritical water extraction (SWE) is a high-efficiency and environment-friendly extraction method. The extraction of resveratrol (RES) of grape seeds obtained from the wine production process was proposed using subcritical water extraction (SWE). The effects of different extraction process parameters on RES yield were investigated by single factors. Extraction optimization was conducted using response surface methodology (RSM). Extraction temperature was proven to be the most significant factor influencing RES yield. The optimal conditions was as follows: extraction pressure of 1.02 MPa, temperature of 152.32 °C, time of 24.89 min, and a solid/solvent ratio of 1:15 g/mL. Under these optimal conditions, the predicted extraction RES yield was 6.90 μg/g and the recoveries was up to 91.98%. Compared to other previous studies, this method required less pollution and less treatment time to extract RES from grape seeds. From these results, added economic value to this agroindustrial residue is proposed using environmentally friendly extraction techniques.
Highlights
Wine industry produces large amounts per year of residues and by-products in form of skins and seeds that are generally untapped
We reported the extraction of resveratrol from grape seeds by subcritical water extraction technique
The results showed that RES yield increased from that of 0.5 MPa: in 1.0 MPa, the yield was highest (6.90 μg/g); from 1.0 MPa to 2.5 MPa, it decreased and intensively decreased during 1.5 MPa to 2.5 MPa
Summary
Wine industry produces large amounts per year of residues and by-products in form of skins and seeds that are generally untapped. Resveratrol is extracted from natural products using organic solvents [12] These techniques require long extraction times and result in low yields of extract. We reported the extraction of resveratrol from grape seeds by subcritical water extraction technique. This method can effectively extract the resveratrol from grape seeds without any organic solvent. The other main objective of this work was to investigate the influence of process parameters (pressure, temperature, time, and solid/solvent ratio) on resveratrol extraction yield from grape seeds. Applying a response surface methodology approach, optimal process conditions for preparation of extracts with the desired observed responses were established It provided a reference of environmentally and human-friendly technologies for the industrial production of resveratrol
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