Abstract
The production of value-added products from lignocellulosic waste has been of great interest for economic as well as environmental concerns. Also, lignocellulosic waste constitutes an attractive renewable feedstock for bioethanol production due to their abundance, availability and low cost. Ethanol production from Manihot esculenta Crantz YTP1 stem was investigated through consolidated bioprocessing (CBP) using Cellulomonas fimi MTCC 24 and Zymomonas mobilis MTCC 92. CBP integrates enzyme production, saccharification (of pre-treated biomass) and fermentation in a single reactor. Screening and optimization of pre-treatment with different acids namely CH3COOH, HNO3, and combined acids (CH3COOH + HNO3) in different dosages, temperature and hydrolysis time, were evaluated for efficient de-lignification of 85.0 ± 3.2% and maximum cellulose release of 87.45 ± 2.2% of biomass. The pre-treatment data obtained were statistically validated using one-way ANOVA. Process parameters such as pH, temperature, agitation and time on the co-production of cellulase and ethanol were statistically optimized by response surface methodology (RSM) and artificial neural network (ANN). The maximum cellulase of 11.63 ± 1.23 IU/mL and ethanol of 9.39 ± 0.33 g/L were obtained in pre-treated biomass. This study demonstrates that M. esculenta Crantz YTP1 stem is an alternative feedstock, and these optimized conditions could be successfully used for commercial production of ethanol using C. fimi MTCC 24 and Z. mobilis MTCC 92 through CBP.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
