Abstract
Generic electromembrane extraction (EME) methods were developed and optimized for basic analytes of moderate or low polarity, employing prototype conductive vial EME equipment. Two generic methods, B1 and B2, were devised for mono- and dibasic compounds with distinct polarity windows: 2.0<log P<6.0 for B1 and 1.0<log P<4.5 for B2. In B1, 10μL of 2-nitrophenyl octyl ether served as the liquid membrane, while B2 utilized 10μL of 2-undecanone. Both methods involved the acidification of 125μL of human plasma samples with 125μL of sample diluent (0.5M HCOOH for B1 and 1.0M HCOOH for B2). The acceptor phase consisted of 250μL of 100mM HCOOH. Extraction was conducted for 30 min with agitation at 800rpm, employing an extraction potential of 100V for B1 and 50V for B2. A set of 90 pharmaceutical compounds was employed as model analytes. Both B1 and B2 demonstrated high recoveries (40%-100%) for the majority of model analytes within their respective polarity windows. Intra-day precision was within 2.2% and 9.7% relative standard deviation. Both extraction systems exhibited stability in terms of current, matrix effect values were between 90% and 109%.
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