Abstract
Statistical experimental designs were used to optimize conditions for recombinant Burkholderia pyrrocinia feruloyl esterase (BpFae) production in bacteria under lactose induction. After optimization by single factor design, Plackett-Burman design, steepest ascent design and the response surface method, the optimal conditions for BpFae production were: 6g/L lactose, pH 5.5, pre-induced period 5h, 23°C, shaker rotational speed of 240rpm, medium volume of 50mL/250mL, inoculum size 0.2% (v/v), and a post-induced period of 32h in a Luria-Bertani culture. The produced BpFae activity was 7.43U/mL, which is 2.92 times higher than that obtained under optimal conditions using IPTG as the inducer. BpFae activity was 4.82U/mL in a 5L fermenter under the abovementioned optimal conditions. BpFae produced a small amount of ethyl acetate but had no effect on the synthesis of other important esters in Baijiu. The results underpin further investigations into BpFae characterization and potential applications.
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