Optimization of Extraction Technology of Total Saponin from Polygonatum sibiricum and Its Inhibitory Activity to α -Amylase and α -Glucosidase

Abstract

Taking the yield of Polygonatum sibiricum saponins as the evaluation index,the cellulase addition amount,pectinase addition amount,material-liquid ratio,enzymatic pH,enzymatic temperature,and enzymatic hydrolysis time were studied by single factor tests. The extraction conditions were optimized,and acarbose was used as a positive control to investigate the inhibitory activities of saponins from Polygonatum sibiricum at different concentrations on α-amylase and α-glucosidase. The results showed that the optimal extraction conditions were:Cellulase addition amount of 0.4%,pectinase addition amount of 5.0%,material-liquid ratio of 1:16 g/mL,enzymolysis pH5.0,enzymolysis temperature 45℃,and enzymolysis time at 2.0 h,the yield of total saponins was 4.06%. When the concentration of Polygonatum sibiricum saponin was 3.000 mg/mL,the highest inhibition rate of α-glucosidase was 74%,which was close to 82% of acarbose(0.5 mg/mL). When the concentration of Polygonatum sibiricum saponin was at 2.000 mg/mL,the highest inhibition rate of α-amylase could reach 82%,exceeding 80% of acarbose(0.5 mg/mL).The compound enzyme method used in this study improved the yield of Polygonatum sibiricum saponins and confirmed that it had certain α-glucosidase and α-amylase inhibitory activities.