Optimization of extraction parameters of biologically active substances from dried biomass of callus, suspension cells and root cultures in vitro

Abstract

Objects of research are the parameters of the extraction of biologically active substances from the dried biomass of callus, suspension cells and root cultures in vitro. The goal of the work was to optimize the parameters of the extraction of biologically active substances from the dried biomass of callus, suspension cells and root cultures in vitro. Organic solvents, a water module, the duration and temperature of the extraction of biologically active substances from the dried biomass of callus, suspension cells and root cultures in vitro were studied. It was found that the optimal parameters for the extraction of biologically active substances from dried biomass of callus cultures of Rhaponticum carthamoides cells are the following: methanol as a solvent, water module 1:10, extraction temperature 60 ° C; for Rhodiola rosea: isopropanol as a solvent, water module 1:10, extraction temperature 60 ° C; for Scutellaria baicalensis: acetone as a solvent, water module 1:10, extraction temperature 50 ° C; for white cinquefoil: ethanol as a solvent, water module 1:20, extraction temperature 40 ° C; for ginseng: acetone as a solvent, water module 1: 5, extraction temperature 50 ° C. The extraction time for all medicinal plants was 60 minutes. The novelty of this work is to optimize existing modes of extraction of biologically active substances from dried biomass of callus, suspension cells and root cultures in vitro for more complete extraction and use.