Abstract
To develop the effective control method for fed-batch culture of cyclosporin A production, we chose fructose, L-valine and (NH4)2HPO4 as feeding nutrients and compared their productivities in relation to different concentrations. The feeding rate of three kinds of feeding materials was controlled to maintain the suitable residual concentration. The fed-batch fermentation results indicated that the optimal concentrations of fructose, L-valine and (NH4)2HPO4 were about 20 g/L, 0.5 g/L and 0.6 g/L for cyclosporin A production, respectively. The cultivation of Beauveria nivea could produce cyclosporin A up to 6.2 g/L for 240 hrs through a continuous feeding-rate-controlled-batch process under the optimal feeding conditions.
Highlights
Cyclosporin A is a cyclic 11-membered fungal peptide metabolite which is widely used as a powerful immunosuppressant in transplantation surgery (Zhao et al, 1991)
The microorganism Tolypocladium inflatum, indicated as Beauveria nivea, was stored in the Agricultural Culture Collection of China (ACCC) as number of 31701.The spores were obtained from a PDA agar, and spore suspensions were prepared after 10 days of cultivation and maintained in 50% glycerol at -80°C
In order to switch the primary metabolism of Beauveria nivea to the phase of cyclosporin A production rapidly and smoothly in fed-batch fermentation, it was necessary to optimize the initial carbon source concentration
Summary
Cyclosporin A is a cyclic 11-membered fungal peptide metabolite which is widely used as a powerful immunosuppressant in transplantation surgery (Zhao et al, 1991). The fungal strain Beauveria nivea was performed to produce cyclosporin A as the matter of continuous fed-batch fermentation. In order to switch the primary metabolism of Beauveria nivea to the phase of cyclosporin A production rapidly and smoothly in fed-batch fermentation, it was necessary to optimize the initial carbon source concentration.
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