Abstract
Microsclerotia (MS) formation was successfully induced in Nomuraea rileyi (Ascomycetes: Hypocreales) in liquid amended medium (AM) culture. To improve MS production, the culture medium was varied. Three quantitative variables, carbon, nitrogen, and basal salt sources, were investigated using the AM. An orthogonal experiment [L9 (3)3] design was applied to optimize the culture medium. Maximum MS production (21.9 × 104 MS ml−1) was obtained using the optimized culture medium (32.0 g l−1 of glucose, 2.0 g l−1 of ammonium citrate, and 0.15 g l−1 of ferrous sulfate). Subsequently, viability, including thermotolerance, shelf-life, and infection, were investigated to evaluate the potential of MS for use as a mycoinsecticide. The MS, collected from the optimized culture medium, exhibited virulence and greater thermotolerance than conidia, and maintained 86.4 % germinability after one-year room temperature storage. These results suggested that the MS obtained through optimization production can be used as a mycoinsecticide.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
