Abstract
In recent years, there has been growing interest in the influence of sprouts on health. Fruit and vegetables are the main sources of organic acids for humans; however, little is yet known about organic acids in sprouts. In this study, the selection of the optimal parameters for extraction of organic acids from fresh, edible sprouts is reported. Two extraction techniques: microwave-assisted (MAE) and ultrasound-assisted were compared. The experimental conditions were optimized in terms of extraction time, temperature, and composition of extraction solution. To determine the influence of time and temperature of extraction or sample cooling, solvents used for extraction, on the analytical signal in isotachophoretic separation, the methods of experimental planning fractional factorial design: 3k−1 were used (three factor, three-level design). The optimal conditions for extraction of organic acids from radish sprouts were MAE, 90 °C; 18 min; and 0.01 M NaOH as a solvent.
Highlights
Conversion of seeds into sprouts through germination results in many biochemical changes
Little is known about the quantity and types of organic acids in different types of vegetable sprouts
It is essential to determine the concentrations of organic acids in edible sprouts in order to be able to classify them as a functional food
Summary
Conversion of seeds into sprouts through germination results in many biochemical changes. Little is known about the quantity and types of organic acids in different types of vegetable sprouts. We have observed progress in the development and application of new techniques for fast and efficient extraction of organic compounds from different plant materials (Alupului et al 2009; Mandal et al 2007; Gallo et al 2010). Microwave-assisted extraction (MAE) is one of the most important techniques used for isolation of various compounds from plants or vegetal materials for both analytical and industrial purposes (Zhang et al 2011; Liu et al 2011; Fang et al 2012). MAE is based on the interaction between microwaves generated by a magnetron or another radiation source and the sampleextracting solvent system
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