Abstract
Objective To select the optimum parameters for amplification of the baculovirus coding for human papillomavirus 18(HPV18)L1 and expression of the corresponding protein.Methods Baculovirus coding for HPVl8 L1 was amplified at different multiplicities of infection(MOls)and time.The virus titers were detected by a viral plaque assay and the optimum parameters were determined.The corresponding protein was expressed at different cell densities,MOIs and time,and was detected and quantified by Western Blot,and the optimum parameters were determined,too.Results The parameters for amplifying the baculovirus and expressing the protein were at the MOI of 0.50 for 48 h and at the cell density of 3×106/ml and MOI of 2.0 for 72 h.Conclusion The optimum parameters for amplifying the baculovirus coding for HPV18 L1 and expressing the corresponding protein are determined. Key words: Human papillomavirus 18; LI gene; Baculovirus; Insect cell; Virus like particle
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
